running wheel manager data acquisition software  (Med Associates Inc)

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Angiotensin‐converting enzyme 2 deficiency accelerates and angiotensin 1‐7 restores age‐related muscle weakness in mice

doi: 10.1002/jcsm.12334

Figure Lengend Snippet: Tissue weight, metabolic function, and physical activity in aged mice. (A) Wet weight of average tibialis anterior (TA) muscle and average gastrocnemius medialis (GM) muscle adjusted by body weight at the age of 3 months ( n = 3–4), 15 months ( n = 5), and 25 months ( n = 8–9). (B) Intraperitoneal glucose tolerance test (ipGTT) was performed by intraperitoneal injection of glucose (2 g/kg body weight) under anaesthesia after 16 h of fasting at the age of 15 months ( n = 6–7) and 25 months ( n = 4–7). Left panel indicates serial change in blood glucose level, and right panel represents area under the curve (AUC). (C) Physical activity was assessed with a running wheel system at the age of 25 months. n = 3–6 in each group. (D) Oxygen consumption (left panel) and carbon dioxide elimination (right panel) were assessed with a metabolic cage at the age of 15 months ( n = 6–7) and 25 months ( n = 7–9). (E) Food consumption during 7 days in 15‐month‐old angiotensin‐converting enzyme 2 knockout (ACE2KO) and wild‐type (WT) mice. Differences between genotypes at the age of 3 and 15 months were analysed with Student's t ‐test. Differences among groups at the age of 25 months were analysed with two‐way ANOVA to detect the difference between the genotypes and the difference between the treatments.

Article Snippet: The amount of exercise was measured using a wireless running wheel system (ENV‐044&SOF‐860; Med Associates Inc., St Albans City, VT, USA).

Techniques: Activity Assay, Injection, Knock-Out